Ssc-miR-185 targets cell division cycle 42 and promotes the proliferation of intestinal porcine epithelial cell.

ABSTRACT:

Objective

microRNAs (miRNAs) can play a role in a variety of physiological and pathological processes, and their role is achieved by regulating the expression of target genes. Our previous high-throughput sequencing found that ssc-miR-185 plays an important regulatory role in piglet diarrhea, but its specific target genes and functions in intestinal porcine epithelial cell (IPEC-J2) are still unclear. We intended to verify the target relationship between porcine miR-185 and cell division cycle 42 (CDC42) gene in IPEC-J2 and to explore the effect of miR-185 on the proliferation of IPEC-J2 cells.

Methods

The TargetScan, miRDB, and miRanda software were used to predict the target genes of porcine miR-185, and CDC42 was selected as a candidate target gene. The CDC423' UTR-wild t

SUBMITTER: Wang W 

PROVIDER: S-EPMC8100468 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature