Capturing T Lymphocytes' Dynamic Interactions With Human Neural Cells Using Time-Lapse Microscopy.

ABSTRACT: To fully perform their functions, T lymphocytes migrate within organs' parenchyma and interact with local cells. Infiltration of T lymphocytes within the central nervous system (CNS) is associated with numerous neurodegenerative disorders. Nevertheless, how these immune cells communicate and respond to neural cells remains unresolved. To investigate the behavior of T lymphocytes that reach the CNS, we have established an in vitro co-culture model and analyzed the spatiotemporal interactions between human activated CD8⁺ T lymphocytes and primary human astrocytes and neurons using time-lapse microscopy. By combining multiple variables extracted from individual CD8⁺ T cell tracking, we show that CD8⁺ T lymphocytes adopt a more motile and exploratory beh

SUBMITTER: Lemaitre F 

PROVIDER: S-EPMC8100528 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature