MYOD1 inhibits avian adipocyte differentiation via miRNA-206/KLF4 axis.

ABSTRACT:

Background

A considerable number of muscle development-related genes were differentially expressed in the early stage of avian adipocyte differentiation. However, the functions of them in adipocyte differentiation remain largely known. In this study, the myoblast determination protein 1 (MYOD1) was selected as a representative of muscle development. We investigated its expression, function, and regulation in avian adipocyte differentiation.

Results

The expression of MYOD1 decreased significantly in the early stage of avian adipocyte differentiation. CRISPR/Cas9-mediated deletion of MYOD1 induced adipocyte differentiation, whereas over-expression of MYOD1 inhibited adipogenesis. The mRNA-seq data showed that MYOD1 could perturb the lipid biosynthetic process during differenti

SUBMITTER: Wang Z 

PROVIDER: S-EPMC8101123 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature