Transgenerational effect of mutants in the RNA-directed DNA methylation pathway on the triploid block in Arabidopsis.

ABSTRACT:

Background

Hybridization of plants that differ in number of chromosome sets (ploidy) frequently causes endosperm failure and seed arrest, a phenomenon referred to as triploid block. In Arabidopsis, loss of function of NRPD1, encoding the largest subunit of the plant-specific RNA polymerase IV (Pol IV), can suppress the triploid block. Pol IV generates short RNAs required to guide de novo methylation in the RNA-directed DNA methylation (RdDM) pathway. Recent work suggests that suppression of the triploid block by mutants in RdDM components differs, depending on whether the diploid pollen is derived from tetraploid plants or from the omission in second division 1 (osd1) mutant. This study aims to understand this difference.

Results

In this study, we find that the ability of mu

SUBMITTER: Wang Z 

PROVIDER: S-EPMC8101200 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature