Embryo CHH hypermethylation is mediated by RdDM and is autonomously directed in Brassica rapa.

ABSTRACT:

Background

RNA-directed DNA methylation (RdDM) initiates cytosine methylation in all contexts and maintains asymmetric CHH methylation. Mature plant embryos show one of the highest levels of CHH methylation, and it has been suggested that RdDM is responsible for this hypermethylation. Because loss of RdDM in Brassica rapa causes seed abortion, embryo methylation might play a role in seed development. RdDM is required in the maternal sporophyte, suggesting that small RNAs from the maternal sporophyte might translocate to the developing embryo, triggering DNA methylation that prevents seed abortion. This raises the question of whether embryo hypermethylation is autonomously regulated by the embryo itself or influenced by the maternal sporophyte.

Results

Here, we demonstrate th

SUBMITTER: Chakraborty T 

PROVIDER: S-EPMC8101221 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature