Proteomic analysis of human frontal and temporal cortex using iTRAQ-based 2D LC-MS/MS.

ABSTRACT:

Background

The human brain is the most complex organ in the body, and it is important to have a better understanding of how the protein composition in the brain regions contributes to the pathogenesis of associated neurological disorders.

Methods

In this study, a comparative analysis of the frontal and temporal cortex proteomes was conducted by isobaric tags of relative and absolute quantification (iTRAQ) labeling and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). Brain protein was taken from relatively normal tissue that could not be avoided of damage during emergent surgery of the TBI (traumatic brain injury) patients admitted in Beijing Tiantan Hospital from 2014 to 2017. Eight cases were included. Four frontal lobes and 4 temporal lobes pro

SUBMITTER: Xu L 

PROVIDER: S-EPMC8101246 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature