Chronic villitis of unknown etiology: Investigations into viral pathogenesis.

ABSTRACT:

Introduction

Chronic villitis of unknown etiology (VUE) is a chronic inflammatory lesion of third trimester placenta, which contributes to major adverse obstetric outcomes. However, the inciting factors and mechanisms by which VUE contributes to adverse outcomes are poorly understood. This limits our ability to develop preventions or interventions. Our goals were to determine whether viruses can be detected in placental tissues with VUE and to determine whether gene expression profiles support an antiviral response.

Methods

We extracted RNA and DNA from 20 placentas with high-grade chronic villitis and 20 control placentas without inflammation. Viruses were assessed using ViroCap viral nucleic acid enrichment coupled with metagenomic sequencing. RNA sequencing was used to ev

SUBMITTER: Ernst LM 

PROVIDER: S-EPMC8101289 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature