Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior.

ABSTRACT: Examining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examining Drosophila melanogaster fru P1 neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use Chromatag to evaluate five chromatin modifications, at three life stages in both sexes. We find substantial changes in chromatin modification profiles across development and fewer differences between males and females. Additionally, we find chromatin modifications that persist in different sets of gene

SUBMITTER: Palmateer CM 

PROVIDER: S-EPMC8102012 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature