RNA-sequencing data-driven dissection of human plasma cell differentiation reveals new potential transcription regulators.

ABSTRACT: Plasma cells (PCs) play an important role in the adaptive immune system through a continuous production of antibodies. We have demonstrated that PC differentiation can be modeled in vitro using complex multistep culture systems reproducing sequential differentiation process occurring in vivo. Here we present a comprehensive, temporal program of gene expression data encompassing human PC differentiation (PCD) using RNA sequencing (RNA-seq). Our results reveal 6374 differentially expressed genes classified into four temporal gene expression patterns. A stringent pathway enrichment analysis of these gene clusters highlights known pathways but also pathways largely unknown in PCD, including the heme biosynthesis and the glutathione conjugation pathways. Additionally, our analysis revealed nume

SUBMITTER: Kassambara A 

PROVIDER: S-EPMC8102200 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature