Rqc1 and other yeast proteins containing highly positively charged sequences are not targets of the RQC complex.

ABSTRACT: Previous work has suggested that highly positively charged protein segments coded by rare codons or poly (A) stretches induce ribosome stalling and translational arrest through electrostatic interactions with the negatively charged ribosome exit tunnel, leading to inefficient elongation. This arrest leads to the activation of the Ribosome Quality Control (RQC) pathway and results in low expression of these reporter proteins. However, the only endogenous yeast proteins known to activate the RQC are Rqc1, a protein essential for RQC function, and Sdd1, a protein with unknown function, both of which contain polybasic sequences. To explore the generality of this phenomenon, we investigated whether the RQC complex controls the expression of other proteins with polybasic sequences. We showed by

SUBMITTER: Barros GC 

PROVIDER: S-EPMC8102910 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature