High-Resolution Regulatory Maps Connect Vascular Risk Variants to Disease-Related Pathways.

ABSTRACT:

Background

Genetic variant landscape of coronary artery disease is dominated by noncoding variants among which many occur within putative enhancers regulating the expression levels of relevant genes. It is crucial to assign the genetic variants to their correct genes both to gain insights into perturbed functions and better assess the risk of disease.

Methods

In this study, we generated high-resolution genomic interaction maps (≈750 bases) in aortic endothelial, smooth muscle cells and THP-1 (human leukemia monocytic cell line) macrophages stimulated with lipopolysaccharide using Hi-C coupled with sequence capture targeting 25 429 features, including variants associated with coronary artery disease. We also sequenced their transcriptomes and mapped putative enhancers using c

SUBMITTER: Akerborg O 

PROVIDER: S-EPMC8104016 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature