Evaluation of a marker independent isolation method for circulating tumor cells in esophageal adenocarcinoma.

ABSTRACT:

Objective

The enrichment of circulating tumor cells (CTCs) from blood provides a minimally invasive method for biomarker discovery in cancer. Longitudinal interrogation allows monitoring or prediction of therapy response, detection of minimal residual disease or progression, and determination of prognosis. Despite inherent phenotypic heterogeneity and differences in cell surface marker expression, most CTC isolation technologies typically use positive selection. This necessitates the optimization of marker-independent CTC methods, enabling the capture of heterogenous CTCs. The aim of this report is to compare a size-dependent and a marker-dependent CTC-isolation method, using spiked esophageal cells in healthy donor blood and blood from patients diagnosed with esophageal adenocarci

SUBMITTER: Philippron A 

PROVIDER: S-EPMC8104412 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature