Hidden Diversity within Common Protozoan Parasites as Revealed by a Novel Genomotyping Scheme.

ABSTRACT: Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is the causative agent of giardiasis, one of the most common diarrheal infections in humans. Evolutionary relationships among G. duodenalis genotypes (or subtypes) of assemblage B, one of two genetic assemblages causing the majority of human infections, remain unclear due to poor phylogenetic resolution of current typing methods. In this study, we devised a methodology to identify new markers for a streamlined multilocus sequence typing (MLST) scheme based on comparisons of all core genes against the phylogeny of whole-genome sequences (WGS). Our analysis identified three markers with resolution comparable to that of WGS data. Using newly designed PCR primers for our novel MLST loci, we typed an add

SUBMITTER: Seabolt MH 

PROVIDER: S-EPMC8105021 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature