Adhesion receptor ADGRG2/GPR64 is in the GI-tract selectively expressed in mature intestinal tuft cells.

ABSTRACT:

Objective

GPR64/ADGRG2 is an orphan Adhesion G protein-coupled receptor (ADGR) known to be mainly expressed in the parathyroid gland and epididymis. This investigation aimed to delineate the cellular expression of GPR64 throughout the body with focus on the gastrointestinal (GI) tract.

Methods

Transgenic Gpr64^mCherry reporter mice were histologically examined throughout the body and reporter protein expression in intestinal tuft cells was confirmed by specific cell ablation. The GPCR repertoire of intestinal Gpr64^mCherry-positive tuft cells was analyzed by quantitative RT-PCR analysis and in situ hybridization. The Gpr64^mCherry was crossed into the general tuft cell reporter Trpm5^GFP to generate small intestinal organoids for time-

SUBMITTER: Grunddal KV 

PROVIDER: S-EPMC8105302 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature