Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil.

ABSTRACT: Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT₇₅. All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT₇₅. In the PRNT₇₅, 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at lea

SUBMITTER: Weber MN 

PROVIDER: S-EPMC8105465 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature