In vitro and in silico anticancer potential analysis of Streptomyces sp. extract against human lung cancer cell line, A549.

ABSTRACT: During our previous investigation, bioactive compounds present in the extract of Streptomyces sp. strain 196 were characterized using LC-MS/MS and ¹H NMR studies. These compounds were K-252-C aglycone indolocarbazole alkaloid, decoyinine, and cycloheximide; the study of these natural drugs against lung carcinoma is still limited. Focus of the current investigation was to study the anticancer effect of strain 196 extract on lung cancer cells (A549). During in vitro studies, anti-proliferative effect of extract was studied using MTT assay in A549 cells. Effect of extract on cell survival was further evaluated using colony assay. Cell death was qualitatively assessed using apoptosis assay. The aftereffect of extract treatment on metastatic potential of cancerous cells was st

SUBMITTER: Kumar P 

PROVIDER: S-EPMC8105467 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature