Comparison of traditional methods and high-throughput genetic sequencing in the detection of pathogens in pulmonary infectious diseases.

ABSTRACT:

Background

The major causes of pulmonary infections are various microorganisms. This study aimed to compare the positive rates of pathogenic microorganism DNA/RNA high-throughput genetic sequencing (PMseq), which is an emerging technique, with traditional methods for pulmonary disease detection, and to investigate the differences in different sample types.

Methods

Bronchoalveolar lavage fluid (BALF) and venous blood samples from 104 patients were collected for detection.

Results

The positive rates of PMseq in BALF and venous blood were both significantly higher than those of traditional methods in the same sample (P<0.001). For BALF, the detection sensitivities were 96.9% for non-febrile patients and 100% for febrile patients. For venous blood, the detection sensitivi

SUBMITTER: Chen X 

PROVIDER: S-EPMC8106068 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature