MiR-194-5p protects against myocardial ischemia/reperfusion injury via MAPK1/PTEN/AKT pathway.

ABSTRACT:

Background

MicroRNA (miRNA), which participates in various physiological and pathological processes, is a highly conserved small RNA sequence. This study aimed to investigate the role of miR-194-5p in hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis and myocardial ischemia/reperfusion (I/R) injury.

Methods

We set up an H/R H9c2 cell model in vitro and an I/R mouse model in vivo. Then, cell vitality, apoptosis, and histopathological evaluation were conducted. Reactive oxygen species (ROS) generation and the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) were examined by 2',7'-Dichlorodihydrofluorescein diacetate (H2DCFDA), and enzyme-linked immunosorbent assay (ELISA), respectively. The level of creatine kinase isoenzyme (CK-MB), car

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC8106072 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature