Assessing nucleic acid binding activity of four dinoflagellate cold shock domain proteins from Symbiodinium kawagutii and Lingulodinium polyedra.

ABSTRACT:

Background

Dinoflagellates have a generally large number of genes but only a small percentage of these are annotated as transcription factors. Cold shock domain (CSD) containing proteins (CSPs) account for roughly 60% of these. CSDs are not prevalent in other eukaryotic lineages, perhaps suggesting a lineage-specific expansion of this type of transcription factors in dinoflagellates, but there is little experimental data to support a role for dinoflagellate CSPs as transcription factors. Here we evaluate the hypothesis that dinoflagellate CSPs can act as transcription factors by binding double-stranded DNA in a sequence dependent manner.

Results

We find that both electrophoretic mobility shift assay (EMSA) competition experiments and selection and amplification binding (SAAB

SUBMITTER: Zaheri B 

PROVIDER: S-EPMC8106185 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature