Controlled modelling of human epiblast and amnion development using stem cells.

ABSTRACT: Early human embryonic development involves extensive lineage diversification, cell-fate specification and tissue patterning¹. Despite its basic and clinical importance, early human embryonic development remains relatively unexplained owing to interspecies divergence^2,3 and limited accessibility to human embryo samples. Here we report that human pluripotent stem cells (hPSCs) in a microfluidic device recapitulate, in a highly controllable and scalable fashion, landmarks of the development of the epiblast and amniotic ectoderm parts of the conceptus, including lumenogenesis of the epiblast and the resultant pro-amniotic cavity, formation of a bipolar embryonic sac, and specification of primordial germ cells and primitive streak cells. We further show that amniotic ectod

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC8106232 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature