Threshold accumulation of a constitutive protein explains E. coli cell-division behavior in nutrient upshifts.

ABSTRACT: Despite a boost of recent progress in dynamic single-cell measurements and analyses in Escherichia coli, we still lack a mechanistic understanding of the determinants of the decision to divide. Specifically, the debate is open regarding the processes linking growth and chromosome replication to division and on the molecular origin of the observed "adder correlations," whereby cells divide, adding roughly a constant volume independent of their initial volume. In order to gain insight into these questions, we interrogate dynamic size-growth behavior of single cells across nutrient upshifts with a high-precision microfluidic device. We find that the division rate changes quickly after nutrients change, much before growth rate goes to a steady state, and in a way that adder correlations are ro

SUBMITTER: Panlilio M 

PROVIDER: S-EPMC8106335 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature