Exploring the oxygenase function of Form II Rubisco for production of glycolate from CO₂.

ABSTRACT: The oxygenase activity of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) converts ribulose-1,5-bisphosphate (RuBP) into 2-phosphoglycolate, which in turn channels into photorespiration, resulting in carbon and energy loss in higher plants. We observed that glycolate can be accumulated extracellularly when two genes encoding the glycolate dehydrogenase of cyanobacteria Synechocystis sp. PCC 6803 were inactivated. This inspired us to explore the oxygenase function of Rubisco for production of glycolate, an important industrial chemical, from CO₂ by engineered cyanobacteria. Since the oxygenase activity of Rubisco is generally low in CO₂-rich carboxysome of cyanobacteria, we introduced Form II Rubisco, which cannot be assembled in carboxysome, into the cytopla

SUBMITTER: Yang F 

PROVIDER: S-EPMC8106553 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature