Differential cellular responses to adhesive interactions with galectin-8- and fibronectin-coated substrates.

ABSTRACT: The mechanisms underlying the cellular response to extracellular matrices (ECMs) that consist of multiple adhesive ligands are still poorly understood. Here, we address this topic by monitoring specific cellular responses to two different extracellular adhesion molecules - the main integrin ligand fibronectin and galectin-8, a lectin that binds β-galactoside residues  - as well as to mixtures of the two proteins. Compared with cell spreading on fibronectin, cell spreading on galectin-8-coated substrates resulted in increased projected cell area, more-pronounced extension of filopodia and, yet, the inability to form focal adhesions and stress fibers. These differences can be partially reversed by experimental manipulations of small G-proteins of the Rho family and their downstream targets,

SUBMITTER: Li W 

PROVIDER: S-EPMC8106957 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature