An electrochemical biosensor for sensitive analysis of the SARS-CoV-2 RNA.

ABSTRACT: The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is continuously worsening globally, herein we have proposed an electrochemical biosensor for the sensitive monitoring of SARS-CoV-2 RNA. The presence of target RNA firstly triggers the catalytic hairpin assembly circuit and then initiates terminal deoxynucleotidyl transferase-mediated DNA polymerization. Consequently, a large number of long single-stranded DNA products can be produced, and these negatively charged DNA products will bind a massive of positively charged electroactive molecular of Ru(NH₃)₆³⁺ due to the electrostatic adsorption. Therefore, significantly amplified electrochemical signals can be generated for sensitive an

SUBMITTER: Peng Y 

PROVIDER: S-EPMC8107000 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature