The role of bone marrow microRNA (miR) in erythropoietic dysfunction after severe trauma.

ABSTRACT:

Background

Previous data has shown that severe traumatic injury is associated with bone marrow dysfunction, which manifests as persistent injury-associated anemia. This study sought to identify whether the expression of erythropoiesis-related microRNAs were altered in the bone marrow of trauma patients to determine if these microRNAs play a role in persistent injury-associated anemia.

Methods

Bone marrow was collected from severely injured trauma patients who underwent fracture fixation as well as patients who underwent elective hip replacement. There were 27 trauma patients and 10 controls analyzed. Total RNA and microRNA were isolated from CD34-positive cells using the RNeasy Plus Mini kit, and genome-wide microRNA expression patterns were assayed. Genes with significant e

SUBMITTER: Apple CG 

PROVIDER: S-EPMC8107112 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature