Lead (Pb) exposure assessment in dried blood spots using Total Reflection X-Ray Fluorescence (TXRF).

ABSTRACT: Lead (Pb) exposure is often determined through the analysis of whole blood though venipuncture poses ethical, economic, and logistical barriers. Dried Blood Spots (DBS) may help overcome such barriers though past studies measuring Pb in DBS have been challenged with quality control, small sample volumes, and other issues. Total Reflection X-Ray Fluorescence (TXRF) may help address some of these challenges but has yet to be used to measure Pb in DBS. As such, the aim of the current study was to develop, validate, and apply a method to analyze Pb in DBS samples using TXRF for use in human biomonitoring studies. First, we developed a novel method (tested a range of parameters), and then used blood reference materials to validate the method against performance criteria listed in ICH Q2A and Q2

SUBMITTER: Rodriguez-Saldana V 

PROVIDER: S-EPMC8107194 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature