Minimum degree of overlap between IL-9R and IL-2R on human T lymphoma cells: A quantitative CLSM and FRET analysis.

ABSTRACT: The heterodimeric receptor complex of IL-9 consists of the cytokine-specific α-subunit and the common γ_c -chain shared with other cytokines, including IL-2, a central regulator of T cell function. We have shown previously the bipartite spatial relationship of IL-9 and IL-2 receptors at the surface of human T lymphoma cells: in addition to common clusters, expression of the two receptor kinds could also be observed in segregated membrane areas. Here we analyzed further the mutual cell surface organization of IL-9 and IL-2 receptors. Complementing Pearson correlation data with co-occurrence analysis of confocal microscopic images revealed that a minimum degree of IL-9R/IL-2R co-localization exists at the cell surface regardless of the overall spatial correlation of the two recepto

SUBMITTER: Nizsaloczki E 

PROVIDER: S-EPMC8108070 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature