Natural abundance isotope ratios to differentiate sources of carbon used during tumor growth in vivo.

ABSTRACT:

Background

Radioactive or stable isotopic labeling of metabolites is a strategy that is routinely used to map the cellular fate of a selected labeled metabolite after it is added to cell culture or to the circulation of an animal. However, a labeled metabolite can be enzymatically changed in cellular metabolism, complicating the use of this experimental strategy to understand how a labeled metabolite moves between organs. These methods are also technically demanding, expensive and potentially toxic. To allow quantification of the bulk movement of metabolites between organs, we have developed a novel application of stable isotope ratio mass spectrometry (IRMS).

Results

We exploit natural differences in ¹³C/¹²C ratios of plant nutrients for a low-cost and

SUBMITTER: Holland P 

PROVIDER: S-EPMC8108461 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature