Differential requirements for the CENP-O complex reveal parallel PLK1 kinetochore recruitment pathways.

ABSTRACT: Similar to other core biological processes, the vast majority of cell division components are essential for viability across human cell lines. However, recent genome-wide screens have identified a number of proteins that exhibit cell line-specific essentiality. Defining the behaviors of these proteins is critical to our understanding of complex biological processes. Here, we harness differential essentiality to reveal the contributions of the four-subunit centromere-localized CENP-O complex, whose precise function has been difficult to define. Our results support a model in which the CENP-O complex and BUB1 act in parallel pathways to recruit a threshold level of PLK1 to mitotic kinetochores, ensuring accurate chromosome segregation. We demonstrate that targeted changes to either pathway s

SUBMITTER: Nguyen AL 

PROVIDER: S-EPMC8108507 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature