Performance of molecular methods for the detection of Salmonella in human stool specimens.

ABSTRACT: Background: The relationship between asymptomatic Salmonella exposure within the gastrointestinal tract and Salmonella bacteraemia is poorly understood, in part due to the low sensitivity of stool culture, and the lack of validated molecular diagnostic tests for the detection of Salmonella in stool. The study aimed to determine a reliable molecular diagnostic test for Salmonella in stool specimens. Methods: We optimized an in-house monoplex real time polymerase chain reaction (PCR) for the detection of SalmonellaTTR and InvA genes in stool by including a selenite broth pre-culture step for Salmonella before DNA extraction, and validated their specificity against other local common pathogens. Then we assessed their performance  against a well-validated multiplex PCR targeting the same TTR a

SUBMITTER: Chirambo AC 

PROVIDER: S-EPMC8108707 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature