ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signaling.

ABSTRACT: ORAI1 and STIM1 are the critical mediators of store-operated Ca ²⁺ entry by acting as the pore subunit and an endoplasmic reticulum-resident signaling molecule, respectively. In addition to Ca ²⁺ signaling, STIM1 is also involved in regulation of a cytosolic nucleic acid sensing pathway. Using ORAI1 and STIM1 knockout cells, we examined their contribution to the host response to SARS-CoV-2 infection. STIM1 knockout cells showed strong resistance to SARS-CoV-2 infection due to enhanced type I interferon response. On the contrary, ORAI1 knockout cells showed high susceptibility to SARS-CoV-2 infection as judged by increased expression of viral proteins and a high viral load. Mechanistically, ORAI1 knockout cells showed reduced homeosta

SUBMITTER: Wu B 

PROVIDER: S-EPMC8109209 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature