Identifying Poly(ADP-ribose)-Binding Proteins with Photoaffinity-Based Proteomics.

ABSTRACT: Post-translational modification of proteins with poly(ADP-ribose) (PAR) is an important component of the DNA damage response. Four PAR synthesis inhibitors have recently been approved for the treatment of breast, ovarian, and prostate cancers. Despite the clinical significance of PAR, a molecular understanding of its function, including its binding partners, remains incomplete. In this work, we synthesized a PAR photoaffinity probe that captures and isolates endogenous PAR binders. Our method identified dozens of known PAR-binding proteins and hundreds of novel candidates involved in DNA repair, RNA processing, and metabolism. PAR binding by eight candidates was confirmed using pull-down and/or electrophoretic mobility shift assays. Using PAR probes of defined lengths, we detected proteins

SUBMITTER: Dasovich M 

PROVIDER: S-EPMC8109396 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature