Highly efficient and automated extraction of DNA from human remains using a modified EZ1 protocol.

ABSTRACT: Bones and teeth often represent the only sources of DNA available for identifying human remains. DNA in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of calcium. Because of the extensive mineralisation, the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction (PCR) inhibitors. Some protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification platform. To improve the efficiency of DNA extraction from skeletal remains, the present study focuses on a modification to these already available protocols. In

SUBMITTER: Barbaro A 

PROVIDER: S-EPMC8110185 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature