Establishment of an anti-inflammation-based bioassay for the quality control of the 13-component TCM formula (Lianhua Qingwen).

ABSTRACT:

Context

Owing to the complexity of chemical ingredients in traditional Chinese medicine (TCM), it is difficult to maintain quality and efficacy by relying only on chemical markers.

Objective

Lianhua Qingwen capsule (LHQW) was selected as an example to discuss the feasibility of a bioassay for quality control.

Materials and methods

Network pharmacology was used to screen potential targets in LHQW with respect to its anti-inflammatory effects. An in vitro cell model was used to validate the prediction. An anti-inflammatory bioassay was established for the quality evaluation of LHQW in 40 batches of marketed products and three batches of destructed samples.

Results

The tumor necrosis factor/interleukin-6 (TNF/IL-6) pathway via macrophage was selected as the

SUBMITTER: Chen S 

PROVIDER: S-EPMC8110188 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature