Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay.

ABSTRACT: Mosquitoes are major infectious disease-carrying vectors. Assessment of current and future risks associated with the mosquito population requires knowledge of the full repertoire of pathogens they carry, including novel viruses, as well as their blood meal sources. Unbiased metatranscriptomic sequencing of individual mosquitoes offers a straightforward, rapid, and quantitative means to acquire this information. Here, we profile 148 diverse wild-caught mosquitoes collected in California and detect sequences from eukaryotes, prokaryotes, 24 known and 46 novel viral species. Importantly, sequencing individuals greatly enhanced the value of the biological information obtained. It allowed us to (a) speciate host mosquito, (b) compute the prevalence of each microbe and recognize a high frequency

SUBMITTER: Batson J 

PROVIDER: S-EPMC8110308 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature