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Transcription-dependent domain-scale three-dimensional genome organization in the dinoflagellate Breviolum minutum.

ABSTRACT: Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state; are not packaged by histones; and contain genes organized into tandem gene arrays, with minimal transcriptional regulation. We analyze the three-dimensional genome of Breviolum minutum, and find large topological domains (dinoflagellate topologically associating domains, which we term 'dinoTADs') without chromatin loops, which are demarcated by convergent gene array boundaries. Transcriptional inhibition disrupts dinoTADs, implicating transcription-induced supercoiling as the primary topological force in dinoflagellates.

SUBMITTER: Marinov GK

PROVIDER: S-EPMC8110477 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Transcription-dependent domain-scale three-dimensional genome organization in the dinoflagellate Breviolum minutum.

Marinov Georgi K GK Trevino Alexandro E AE Xiang Tingting T Kundaje Anshul A Grossman Arthur R AR Greenleaf William J WJ

Nature genetics 20210429 5

Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state; are not packaged by histones; and contain genes organized into tandem gene arrays, with minimal transcriptional regulation. We analyze the three-dimensional genome of Breviolum minutum, and find large topological domains (dinoflagellate topologically associating domains, which we term 'dinoTADs') without chromatin loops, which are demarcated by convergent gen ...[more]

PMID: 33927397

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Transcription-dependent domain-scale 3D genome organization in dinoflagellates

Project description:Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state, are not packaged by histones, and contain genes organized into polycistronic arrays, with minimal transcriptional regulation. We analyze the 3D genome of {Breviolum minutum}, and find large topological domains without chromatin loops, demarcated by convergent gene array boundaries (``dinoTADs’’). Transcriptional inhibition degrades dinoTADs, implicating transcription-induced supercoiling as the primary topological force in dinoflagellates.

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