Identification of novel pentose transporters in Kluyveromyces marxianus using a new screening platform.

ABSTRACT: The capacity of yeasts to assimilate xylose or arabinose is strongly dependent on plasma membrane transport proteins. Because pentoses comprise a substantial proportion of available sugars in lignocellulosic hydrolysates, their utilisation is centrally important for the development of second generation biorefineries. Relatively few native pentose transporters have been studied and there is intense interest in expanding the repertoire. To aid the identification of novel transporters, we developed a screening platform in the native pentose-utilising yeast Kluyveromyces marxianus. This involved the targeted deletion of twelve transporters of the major facilitator superfamily (MFS) and application of a synthetic biology pipeline for rapid testing of candidate pentose transporters. Using this K

SUBMITTER: Donzella L 

PROVIDER: S-EPMC8110514 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature