A TILLING by sequencing approach to identify induced mutations in sunflower genes.

ABSTRACT: The Targeting Induced Local Lesions in Genomes (TILLING) technology is a reverse genetic strategy broadly applicable to every kind of genome and represents an attractive tool for functional genomic and agronomic applications. It consists of chemical random mutagenesis followed by high-throughput screening of point mutations in targeted genomic regions. Although multiple methods for mutation discovery in amplicons have been described, next-generation sequencing (NGS) is the tool of choice for mutation detection because it quickly allows for the analysis of a large number of amplicons. The aim of the present work was to screen a previously generated sunflower TILLING population and identify alterations in genes involved in several important and complex physiological processes. Twenty-one can

SUBMITTER: Fanelli V 

PROVIDER: S-EPMC8110748 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature