Improved Microbial Community Characterization of 16S rRNA via Metagenome Hybridization Capture Enrichment.

ABSTRACT: Environmental microbial diversity is often investigated from a molecular perspective using 16S ribosomal RNA (rRNA) gene amplicons and shotgun metagenomics. While amplicon methods are fast, low-cost, and have curated reference databases, they can suffer from amplification bias and are limited in genomic scope. In contrast, shotgun metagenomic methods sample more genomic regions with fewer sequence acquisition biases, but are much more expensive (even with moderate sequencing depth) and computationally challenging. Here, we develop a set of 16S rRNA sequence capture baits that offer a potential middle ground with the advantages from both approaches for investigating microbial communities. These baits cover the diversity of all 16S rRNA sequences available in the Greengenes (v. 13.5) databas

SUBMITTER: Beaudry MS 

PROVIDER: S-EPMC8110821 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature