Fabrication of PNIPAm-based thermoresponsive hydrogel microwell arrays for tumor spheroid formation.

ABSTRACT: Complex three-dimensional (3D) cell cultures are being increasingly implemented in biomedical research as they provide important insights into complex cancer biology, and cell-cell and cell-matrix interactions in the tumor microenvironment. However, most methods used today for 3D cell culture are limited by high cost, the need for specialized skills, low throughput and the use of unnatural culture environments. We report the development of a unique biomimetic hydrogel microwell array platform for the generation and stress-free isolation of cancer spheroids. The poly N-isopropylacrylamide-based hydrogel microwell array (PHMA) has thermoresponsive properties allowing for the attachment and growth of cell aggregates/ spheroids at 37 °C, and their easy isolation at room temperature (RT). The r

SUBMITTER: Dhamecha D 

PROVIDER: S-EPMC8110948 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature