LoReTTA, a user-friendly tool for assembling viral genomes from PacBio sequence data.

ABSTRACT: Long-read, single-molecule DNA sequencing technologies have triggered a revolution in genomics by enabling the determination of large, reference-quality genomes in ways that overcome some of the limitations of short-read sequencing. However, the greater length and higher error rate of the reads generated on long-read platforms make the tools used for assembling short reads unsuitable for use in data assembly and motivate the development of new approaches. We present LoReTTA (Long Read Template-Targeted Assembler), a tool designed for performing de novo assembly of long reads generated from viral genomes on the PacBio platform. LoReTTA exploits a reference genome to guide the assembly process, an approach that has been successful with short reads. The tool was designed to deal with r

SUBMITTER: Al Qaffas A 

PROVIDER: S-EPMC8111061 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature