High-Throughput Screening Platform for Nanoparticle-Mediated Alterations of DNA Repair Capacity.

ABSTRACT: The potential genotoxic effects of engineered nanomaterials (ENMs) may occur through the induction of DNA damage or the disruption of DNA repair processes. Inefficient DNA repair may lead to the accumulation of DNA lesions and has been linked to various diseases, including cancer. Most studies so far have focused on understanding the nanogenotoxicity of ENM-induced damages to DNA, whereas the effects on DNA repair have been widely overlooked. The recently developed fluorescence multiplex-host-cell reactivation (FM-HCR) assay allows for the direct quantification of multiple DNA repair pathways in living cells and offers a great opportunity to address this methodological gap. Herein an FM-HCR-based method is developed to screen the impact of ENMs on six major DNA repair pathways using suspen

SUBMITTER: Toprani SM 

PROVIDER: S-EPMC8111687 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature