Physiologically relevant oxygen tensions differentially regulate hepatotoxic responses in HepG2 cells.

ABSTRACT: This study evaluates the impact of physiologically relevant oxygen tensions on the response of HepG2 cells to known inducers and hepatotoxic drugs. We compared transcriptional regulation and CYP1A activity after a 48 h exposure at atmospheric culture conditions (20% O₂) with representative periportal (8% O₂) and perivenous (3% O₂) oxygen tensions. We evaluated cellular responses in 2D and 3D cultures at each oxygen tension in parallel, using monolayers and a paper-based culture platform that supports cells suspended in a collagen-rich environment. Our findings highlight that the toxicity, potency, and mechanism of action of drugs are dependent on both culture format and oxygen tension. HepG2 cells in 3D environments at physiologic oxygen tensions better mat

SUBMITTER: DiProspero TJ 

PROVIDER: S-EPMC8111698 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature