Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysis.

ABSTRACT:

Background

High throughput single-cell RNA sequencing (scRNA-Seq) has emerged as a powerful tool for exploring cellular heterogeneity among complex human cancers. scRNA-Seq studies using fresh human surgical tissue are logistically difficult, preclude histopathological triage of samples, and limit the ability to perform batch processing. This hindrance can often introduce technical biases when integrating patient datasets and increase experimental costs. Although tissue preservation methods have been previously explored to address such issues, it is yet to be examined on complex human tissues, such as solid cancers and on high throughput scRNA-Seq platforms.

Methods

Using the Chromium 10X platform, we sequenced a total of ~ 120,000 cells from fresh and cryopreserved replicat

SUBMITTER: Wu SZ 

PROVIDER: S-EPMC8111910 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature