Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum.

ABSTRACT:

Background

The demand for biobased polymers is increasing steadily worldwide. Microbial hosts for production of their monomeric precursors such as glutarate are developed. To meet the market demand, production hosts have to be improved constantly with respect to product titers and yields, but also shortening bioprocess duration is important.

Results

In this study, adaptive laboratory evolution was used to improve a C. glutamicum strain engineered for production of the C₅-dicarboxylic acid glutarate by flux enforcement. Deletion of the L-glutamic acid dehydrogenase gene gdh coupled growth to glutarate production since two transaminases in the glutarate pathway are crucial for nitrogen assimilation. The hypothesis that strains selected for faster glutarate-coupled g

SUBMITTER: Prell C 

PROVIDER: S-EPMC8112011 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature