Pannexin-1 Contributes to the Apoptosis of Spinal Neurocytes in Spinal Cord Injury.

ABSTRACT: Currently, the role of Pannexin-1, a homomeric membrane hemichannel on the neuron cell membrane, in the development of spinal cord injury (SCI) is largely unknown. Herein, we assessed the contribution of Panx1 in the development of SCI. The SCI in vitro model was established using rat primary spinal neurocytes treated with hydrogen peroxide (H₂O₂). Effects of Panx1 overexpression or depletion in spinal neurocytes were analyzed by lentivirus-mediated transfection of Panx1 and interference sh-Panx1. Decreased cell viability was seen in SCI cells, which was further enhanced under Panx1 overexpression and mitigated by Panx1 deficiency. H₂O₂ induced an increase of intracellular Ca²⁺ signal and upregulated level of the proapoptotic pr

SUBMITTER: Huang Y 

PROVIDER: S-EPMC8112589 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature