A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus.

ABSTRACT: Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. It demonstrated its capability to discover key regulatory elements that determine cell fate during developmental programs. Single-cell analysis requires the isolation and labeling of the messenger RNA (mRNA) derived from each cell. These challenges were primarily addressed in mammals by developing microfluidic-based approaches. For plant species whose cells contain cell walls, these approaches have generally required the generation of isolated protoplasts. Many plant tissues' secondary cell wall hinders enzymatic digestion required for individual protoplast isolation, resulting in an unequal representa

SUBMITTER: Conde D 

PROVIDER: S-EPMC8112699 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature