Circadian time series proteomics reveals daily dynamics in cartilage physiology.

ABSTRACT:

Objective

Cartilage in joints such as the hip and knee experiences repeated phases of heavy loading and low load recovery during the 24-h day/night cycle. Our previous work has shown 24 h rhythmic changes in gene expression at transcript level between night and day in wild type mouse cartilage which is lost in a circadian clock knock-out mouse model. However, it remains unknown to what extent circadian rhythms also regulate protein level gene expression in this matrix rich tissue.

Methods

We investigated daily changes of protein abundance in mouse femoral head articular cartilage by performing a 48-h time-series LC-MS/MS analysis.

Results

Out of the 1,177 proteins we identified across all time points, 145 proteins showed rhythmic changes in their abundance within the

SUBMITTER: Dudek M 

PROVIDER: S-EPMC8113022 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature