Wnt Signaling Mediates Pro-Fibrogenic Activity in Human Aortic Valve Interstitial Cells.

ABSTRACT:

Background

Proinflammatory activation of toll-like receptor-4 (TLR4) drives phenotypic changes in aortic valve interstitial cells (AVICs) and produces a fibrogenic phenotype that mediates valvular fibrosis and contributes to aortic stenosis. Prior work identified upregulated Wnt signaling in AVICs taken from valves affected by aortic stenosis. Our purpose was to determine the contribution of Wnt signaling to TLR4-dependent fibrogenic activity in isolated human AVICs.

Methods

Human AVICs were isolated from hearts explanted for cardiac transplantation (N = 4). To test whether Wnt signaling contributed to TLR4-dependent fibrogenic activity, AVICs were treated with Wnt inhibitor (Dkk1) prior to TLR4 activation (LPS) and fibrogenic markers assessed. To determine the mediator of T

SUBMITTER: Jarrett MJ 

PROVIDER: S-EPMC8113342 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature