Phenotypic variation of transcriptomic cell types in mouse motor cortex.

ABSTRACT: Cortical neurons exhibit extreme diversity in gene expression as well as in morphological and electrophysiological properties^1,2. Most existing neural taxonomies are based on either transcriptomic^3,4 or morpho-electric^5,6 criteria, as it has been technically challenging to study both aspects of neuronal diversity in the same set of cells⁷. Here we used Patch-seq⁸ to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse primary motor cortex, providing a morpho-electric annotation of almost all transcriptomically defined neural cell types. We found that, although broad families of transcriptomic types (those expressing Vip, Pvalb, Sst and so on) had distinct and essenti

SUBMITTER: Scala F 

PROVIDER: S-EPMC8113357 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature